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降解黄曲霉毒素B1土曲霉发酵工艺优化的研究(网络首发、推荐阅读)
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Study on Optimization of Fermentation Process of Aflatoxin B1 Degradation by Aspergillusterreus(Online First, Recommended Article)
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    摘要:

    对黄曲霉毒素B1(Aflatoxin B1,AFB1)降解菌土曲霉(Aspergillusterreus)HNGD-TM15降解AFB1发酵工艺进行优化。在单因素实验基础上,运用Box-Behnken设计原理,对降解AFB1影响显著的HNGD-TM15发酵工艺参数碳源(葡萄糖)、pH和温度设计响应面实验,结果表明:模型(P=0.002 8)在1%水平上具有极显著性,失拟项不显著(P=0.063 5>0.05),其R2=0.929 9,R2Adj=0.839 9,说明该模型拟合程度良好,可以模拟92.99%的AFB1降解率变化,其中pH对AFB1降解率的影响最大。确定HNGD-TM15发酵主要参数:葡萄糖0.7%,发酵液起始pH为3.0,发酵温度为34 ℃,接种量为0.006%的菌悬液,装液量为100 mL/250 mL,发酵时间为72 h。优化后AFB1降解率从接种量为5%的98.30%提高到接种量为0.006%的99.94%。

    Abstract:

    The fermentation process of Aflatoxin B1(Aflatoxin B1, AFB1) degrading bacteria Aspergillusterreus HNGD-TM15 to degrade AFB1 was optimized. Based on the single-factor experiment, the Box-Behnken design principle was used to design the response surface experiment for the HNGD-TM15 fermentation process parameters, including carbon source (glucose), pH and temperature, which can significantly affect the degradation of AFB1. The results showed that the model (P=0.002 8) was extremely significant at 1% level, and the lack-of-fit term is not significant (P=0.063 5>0.05) with R2=0.929 9 and R2Adj=0.839 9, indicating that the model fits well, and it can simulate 92.99% of AFB1 degradation rate changes, in which pH has the greatest effect on the degradation rate of AFB1. The fermentation parameters of HNGD-TM15 were determined as follows: glucose was 0.7%, the initial pH of the fermentation broth was 3.0, the fermentation temperature was 34 ℃, the inoculation volume was 0.006% of the bacterial suspension, and the filling volume was 100 mL/250 mL, fermentation time was 72 h. After optimization, the degradation rate of AFB1 was increased from 98.30% with an inoculation volume of 5% to 99.94% with an inoculation volume of 0.006%.

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宁梦鸽,谢岩黎*,孙淑敏,马卫宾,李 倩,卫 敏,何保山,任文洁.降解黄曲霉毒素B1土曲霉发酵工艺优化的研究(网络首发、推荐阅读)[J].粮油食品科技,2020,28(5):18-23.

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  • 在线发布日期: 2020-09-16
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