In order to explore the research hotspots and development trends of protein deamidation, this paper systematically searched 133 Chinese literatures and 337 English literatures on protein deamidation in the CNKI and WOS databases, and the CiteSpace software was used to draw knowledge graphs and visual analysis of publication trends, author collaboration networks, national collaboration networks, institutional cooperation network, keywords and other content. The results showed that the volume of Chinese publications was relatively low and showed a steady trend, while the volume of English publications showed a wave-like upward trend, with a rapid increase from 2020 to 2023, exceeding 30 publications per year. In addition, both Chinese and English literature formed relatively fixed author clusters with close collaborations within the groups, but limited collaborations between clusters. The research hotspots in this field were generally consistent domestically and internationally, mainly focusing on the influence of deamidation on the structure and functional characteristics of proteins. Some research has also been conducted on the mechanisms underlying the influence on functional characteristics and the sensitization of proteins. In recent years, the application of protein glutaminase in the field of protein deamidation has become a new research hotspot.