The results of resistance characteristics in different grains during ventilation experiments showed that the resistance of unit grain layer was positively correlated with the surface wind speed of grain, and the polynomial quadratic function had the higher fitting degree for them. When the surface wind speed of grain was at the same level, the resistance of unit grain layer in soybean was lower, followed by corn but that of rice and wheat was higher, indicating that the resistance of unit grain layer was related to grain structure、surface smoothness and porosity. The resistance of net was positively correlated with the surface wind speed of grain, with the increase of surface wind speed and the resistance of net during ventilation process. The resistance of net could increase sharply when the surface wind speed increased to a certain extent. In order to avoid the waste of energies, the large volume shouldn’t choose blindly during the process of ventilation. The ventilation uniformity of grain increased gradually with the increase of the grain layer thickness, which was less affected by the ventilation volume when the ventilation uniformity of different grains was compared, rice was lower, follow by wheat, and soybean and corn was higher, which was related to grain shape and stacking structure.