By investigating the absolute sensitivity and maximum dilution ratio of commercially available kits, three kinds of rapid detection kit products with the best effect were selected. Fried peanuts, roasted peanuts and peanut butter were taken as representative substrates to optimize the pre-treatment conditions of samples and the reaction conditions of kits. Unified technical evaluation was conducted according to the performance indexes in The Technical Specification for Evaluation of Rapid Food Detection Methods, and the detection limit, sensitivity, specificity, false negative rate, false positive rate, relative accuracy and consistency analysis of reference methods of all brands of rapid test kits were investigated. Our findings could provide scientific basis for the rapid determination and evaluation standard of aflatoxin B1 colloidal gold in peanut and its products.