Resistant starch has the benefits in the regulation of intestinal flora, improvement of intestinal function, control of cholesterol levels and glycemic index. In recent years, the most concerned is the prebiotic effect of resistant starch. Fermented milk has been considered as the best carrier of probiotics and prebiotics. Adding type III resistant starch to fermented milk could not only improve the quality of fermented milk, but also enhance the functionality of fermented milk. This paper briefly introduced the relationships between resistant starch and prebiotics, and summarized the application of type III resistant starch in fermented milk. This paper also expounded the effect of type III resistant starch on the quality of fermented milk and its probiotic evaluation, which could provide reference for the application and development of type III resistant starch in functional fermented milk.