Through the research on the determination of bisphenol A in vegetable oil by high-performance liquid chromatography, this article established the method for determining bisphenol A in vegetable oil. Bisphenol A was extracted with acetonitrile.The extract was purified and eluted by the amino solid-phase extraction column and then concentrated and reconstituted. After filtration, the extract was separated by high-performance liquid chromatography, detected by the fluorescence detector, and quantified by the standard external method. The methodological performance analysis showed that the linear range was 0.003 333~0.666 7 μg/mL, the correlation coefficient (r) of the standard curve is greater than 0.999, the detection limit of the method is 0.007 mg/kg, see the quantification limit was 0.013 mg/kg, the recoveries ranged from 90.8% to 103.2%, and the coefficient of variation of seven replicate assays was 2.8%. It was verified that the method had good linearity, high sensitivity, good repeatability, accurate and reliable results, and could meet the requirements of GB/T 27417—2017, providing a technical basis for the scientific evaluation of the content of bisphenol A in vegetable oil.