This research confirmed the indicator and its weight for constructing the network of the food safety grass-roots responsibility, established the evaluation indicator system and performed empirical analysis. By combining with the “three sets” scheme of food safety grass-roots responsibility network and local standards, and using the balanced scorecard theory, we determined the evaluation index system by AHP, and carried out the evaluation in 11 regions of Zhejiang Province. The evaluation system consisted of 4 first-level indicators, 11 second-level indicators and 27 third-level indicators. The weights of first-level indicators from high to low were performance evaluation (0.512 7), internal management (0.260 5), related stakeholders (0.150 4), and learning and growth (0.076 4). The top three third-level indicators were high public satisfaction (0.244 9), resource security (0.138 3) and no food safety incidents (0.099 3). The evaluation results of the order of 11 regions were consistent with the evaluation results of Zhejiang. The evaluation index system was feasible, effective, scientific and reasonable, thus could provide direction for the construction of food safety grass-roots responsibility network in various places, and provide scientific decision-making and objective basis for the evaluation of the performance of the responsibility network.