This research studied the effect of cordyceps militaris noodles on alleviating physical fatigue and concentration of serum lipids in mouse. The mouse were divided into feed group, blank noodle group, low dose of C.militaris noodles group and high dose of C.militaris noodles group, and the animal model of swimming fatigue was established. The exhausted swimming time of mouse in each group was recorded, and the related indexes of alleviating physical fatigue and affecting lipid concentration in mouse were measured. The results show that there was no significant difference in body weight of mouse in each group. Compared with feed group and blank noodle group, cordyceps militaris noodles could effectively prolong the exhausted swimming time of mouse (P<0.01), and the average exhausted swimming time of mouse in high dose group was 3 817.50 s, which was 2.33 times of that in feed group and 2.35 times of that in blank noodle group. The adding of high dose of C.militaris to noodles significantly increased the contents of lactate dehydrogenase and high-density lipoprotein, increased the storage of glycogen, inhibited the accumulation of urea nitrogen in serum, and decreased the contents of triglyceride and total cholesterol (P <0.05). C.militaris noodles had no effect on normal growth and development of mouse, and could significantly relieve physical fatigue and maintain blood lipid concentration in vivo.