Lipase TLIM was used as catalyst to prepare low erucic acid glyceride from Xanthoceras sorbifolia Bunge oil. The effects of enzyme addition amount, reaction time, reaction temperature and water/oil molar ratio on hydrolysis reaction were investigated by single factor test, and the hydrolysis reaction conditions were optimized by orthogonal test. The results showed that the optimal reaction conditions for the preparation of low erucic acid triglyceride from Xanthoceras sorbifolia Bunge catalyzed by immobilized lipase TLIM were as follows: enzyme dosage of 5%, reaction temperature of 45 ℃, reaction time of 5 h, water/oil ratio of 30∶1. Under these conditions, the content of erucic acid in Xanthoceras sorbifolia Bunge triglyceride reached 4.86%. It was proved that erucic acid of Xanthoceras sorbifolia Bunge oil was distributed in position 1 and 3 of triglyceride, and the content of nervonic acid was kept at about 1%.