粮油食品科技

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两株高效降解玉米蛋白粉益生菌的发酵特性研究
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Research on Fermentation Characteristics of Two Probiotic Strains with Efficient Degradation of Corn Gluten Meal
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    通过固体筛培法从豆酱样品筛选到两株高效降解玉米蛋白粉的菌株Bacillus subtilis HDJ1和Bacillus velezensis HDJ2。单因素发酵实验表明,菌株HDJ1液态发酵玉米蛋白粉的最佳条件为:发酵温度40 ℃,pH 8,接种量6%,底物浓度8%,转速200 r/min,加液体积40 mL,发酵时间72 h;菌株HDJ2液态发酵玉米蛋白粉的最优条件为:发酵温度40 ℃,pH 6,接种量9%,底物浓度8%,转速150 r/min,加液体积120 mL,发酵时间72 h;HDJ1和HDJ2等量组合后发酵玉米蛋白粉的最佳条件为:发酵温度30 ℃,发酵pH 8,接种量6%,底物浓度8%,转速250 r/min,加液体积40 mL,发酵时间96 h。正交实验结果显示:HDJ1、HDJ2、HDJ1和HDJ2等量组合在发酵温度30 ℃,发酵pH 8,接种量3%,底物浓度8%,转速200 r/min,发酵时间96 h条件下发酵液可溶蛋白含量最高,其发酵液冷冻干燥浓缩样品的蛋白酶活、可溶蛋白含量、DPPH清除率等显著提高,且两益生菌菌株组合后的发酵效果优于单株菌。

    Abstract:

    Two microbial strains Bacillus subtilis HDJ1 and Bacillus velezensis HDJ2 were screened from soybean paste samples by corn gluten meal medium for high protease activity. Single factor fermentation experiment showed that the optimum conditions of liquid fermentation of corn gluten meal for strain HDJ1 were: fermentation temperature of 40 ℃, fermentation pH 8, inoculum of 6%, substrate concentration of 8%, rotating speed of 200 r/min, liquid-adding volume of 40 mL, fermentation time of 72 h; The optimum conditions for liquid fermentation of corn gluten meal for strain HDJ2 were: fermentation temperature of 40 ℃, fermentation pH 6, inoculum of 9%, substrate concentration of 8%, rotating speed of 150 r/min, liquid-adding volume of 120 mL, fermentation time of 72 h; The optimum conditions for the fermentation of corn gluten meal for equal combination of HDJ1 and HDJ2 were: fermentation temperature of 30 ℃, fermentation pH 8, inoculum amount of 6%, substrate concentration of 8%, rotating speed of 250 r/min, liquid-adding volume of 40 mL, fermentation time of 96 h. The results of Orthogonal test showed that the content of soluble protein of HDJ1, HDJ2, HDJ1 and HDJ2 was the highest when the fermentation temperature was 30 ℃, the fermentation pH was 8, the inoculum amount was 3%, the substrate concentration was 8%, therotation speed was 200 r/min, and the fermentation time was 96 h. The protease activity, soluble protein content and DPPH scavenging rate of the sample significantly increased after freeze-drying of the microbial fermentation, and the fermentation effect of the two combined stains was better than that of single strain.

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任 菲,刘玉春,王 超,郭 超,张维清.两株高效降解玉米蛋白粉益生菌的发酵特性研究[J].粮油食品科技,2022,30(2):183-189.

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  • 在线发布日期: 2022-03-24
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