In this paper, tofu and plant ash were used as the main raw materials to prepare the plant ash culture tofu by ash culture, marinating and frying. The best formula of the plant ash culture tofu was determined by single factor and orthogonal experiment. The results showed that: the best formula of the plant ash culture tofu was as follows: the ash culture time of tofu was 6 days, adding 2.2% salt, 0.6% chili powder, 2% soy sauce, 1% sugar, 0.04% black pepper, 0.5% monosodium glutamate and 0.2% five spice powder to every 1000 milliliter soup. The prepared ash culture tofu has light soy sauce color, soft texture, moderate salty and spicy, and strong bean flavor. Compared with ordinary tofu, the hardness, elasticity, stickiness and chewiness of ash culture tofu were 5.17 times, 1.33 times, 6.24 times and 8.78 times of ordinary tofu, respectively.