V79 cells were exposed to whole cigarette smoke for 1 hour with 4 cigarettes per hour, 6 cigarettes per hour and 8 cigarettes per hour respectively. ROS, SOD, GSH-Px, CAT and T-AOC were taken as index, and the effects of cigarette smoke on the antioxidant capacity of V79 cells were studied. The results showed that the whole smoke environment will consume and inhibit the antioxidant substance in the cell, which leads to the increase of the content of active oxygen free radical in cells.