In order to explore an environmental removal method of grain mycotoxins and to overcome the problem of poor stability of biodegradation method, the Tibetan yeast strains were selected as the research object, and the strains with better degradation effects on zearalenone (ZEN) were screened to explore the functions and mechanisms in this study. The results showed that Tibet Rhodotorula mucilaginosa had a good degradation effect on ZEN, and the degradation effect gradually improved with the increase of the bacterial concentration. Through the evaluation of the toxin removal effect of different yeast treatment solutions, it was found that the removal mechanism of the yeast on ZEN was mainly adsorption, accompanied by a certain degree of intracellular biodegradation. The degradation products of ZEN were determined by liquid chromatography-mass spectrometry. It was found that the degradation products may be zearalenol and zearalenone, and the safety of the degradation products needed further verification. The stress tolerance test of Tibet R. mucilaginosa showed that it had a certain tolerance to low temperature and oxidative stress.