The purpose of this study was to prepare a kind of Zearalenone fast quantitative test strip based on time-resolved fluorescence immunochromatographic assay. Europium nanoparticles were used as fluorescent probes to label anti-zearalenone monoclonal antibodies, and a quantitative immunochromatography method was established based on competitive inhibition principle. The results showed that the sensitivity of Zearalenone fluorescent detection strip was 0.1 ng/mL, the linear range was 0.1~5.0 ng/mL, the standard recovery rate of negative samples of corn and wheat was between 93.45% and 112.2%, and the coefficient of variation of 5 repeats for the same sample was less than 15%. The results show that the Zearalenone fluorescent test strip has the technical characteristics of high sensitivity, short reaction time and accurate quantification, which is suitable for the rapid quantitative detection of Zearalenone in grain and its products.