To study the immunomodulatory effect of jujube polysaccharide on mouse lymphocytes. Mouse lymphocytes were sterile separated, cell suspension was prepared, and the blank group, the positive group (levamisole) and the jujube polysaccharide group with different mass concentrations (final concentrations of 20, 40, 80, 160 and 320 living μg/ml) were designed. The effect of jujube polysaccharide on the proliferation of mouse lymphocytes was observed by MTT method. The effects of jujube polysaccharide on the levels of interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-10 (IL-10) and interleukin-12 (IL-12) in the supernatant of mouse lymphocytes were observed by ELISA. The effect of jujube polysaccharide on the expression of IL-2, IL-6, IL-10 and IL-12 mRNA in mouse lymphocytes was detected by QRT-PCR. Jujube polysaccharide could promote the proliferation of mouse lymphocytes and the secretion of cytokines in the range of 20-320 μg/ml, which further promoted the expression of cytokines IL-2 and IL-6, as well as IL-10 and IL-12 mRNA. Conclusion: jujube polysaccharide enhances immune function by inducing lymphocyte proliferation and the secretion and mRNA expression of lymphocyte cytokines IL-2 and IL-6, as well as IL-10 and IL-12.