Jatropha crude oil and compound phospholipase were used as raw material and degumming enzyme respectively, the enzymatic degumming process was studied by single factor test and orthogonal array test. The results showed that the optimal conditions were confirmed as follows: enzyme dosage, 50 μg/g; pH5.5; reaction temperature, 50 ℃, stirring speed, 350 r/min; reaction time, 2 h. The contents of phosphorus, metal, nitrogen and other trace impurities were significantly reduced in degumming oil, and the degumming effect was obvious. On the basis of test research, enzymatic degumming mechanism of crude oil was also analyzed.