As the environmental protection has been more and more concerned nowadays, biological pesticides have become a research focus by pesticide researches all over the world due to their advantages of easy degradation, low residue, low pollution, high safety and low generation of resistance in diseases and insect pests, and the development of biological pesticides has become a new trend in leading pesticide research and development. As a new type of green biological pesticide, spinosyns and their analogues have a unique action mechanism due to their special chemical structure. They have both the safety of biological pesticides and the rapid effect of chemical pesticides and play an important role in controlling agricultural and forestry pests, stored grain pests, sanitary pests, and livestock parasites. This study mainly compared the physicochemical properties of biopesticide spinosyns and their analogues, summarized the progress in physicochemical mutagenesis, molecular modification, product research and development and application, and resistance research, analyzed the current situation and existing problems of biological pesticides promotion, proposed solutions, and looked forward to their development prospects.