Abstract:The fermentation process of Aflatoxin B1(Aflatoxin B1, AFB1) degrading bacteria Aspergillusterreus HNGD-TM15 to degrade AFB1 was optimized. Based on the single-factor experiment, the Box-Behnken design principle was used to design the response surface experiment for the HNGD-TM15 fermentation process parameters, including carbon source (glucose), pH and temperature, which can significantly affect the degradation of AFB1. The results showed that the model (P=0.002 8) was extremely significant at 1% level, and the lack-of-fit term is not significant (P=0.063 5>0.05) with R2=0.929 9 and R2Adj=0.839 9, indicating that the model fits well, and it can simulate 92.99% of AFB1 degradation rate changes, in which pH has the greatest effect on the degradation rate of AFB1. The fermentation parameters of HNGD-TM15 were determined as follows: glucose was 0.7%, the initial pH of the fermentation broth was 3.0, the fermentation temperature was 34 ℃, the inoculation volume was 0.006% of the bacterial suspension, and the filling volume was 100 mL/250 mL, fermentation time was 72 h. After optimization, the degradation rate of AFB1 was increased from 98.30% with an inoculation volume of 5% to 99.94% with an inoculation volume of 0.006%.