粮油食品科技

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基于免疫磁珠黄曲霉毒素全自动净化方法适用性评价
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Evaluation of the applicability of an automatic method for aflatoxin purification based on immunomagnetic beads
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    对基于免疫磁珠黄曲霉毒素全自动净化的方法适用性进行研究。结果表明:免疫磁珠试剂盒平均回收率均在95%以上,相对标准偏差小于3.3%。质控样品的测定结果均在给参考值范围内。通过t检验可知,sig.(双侧)=0.533,两台净化仪的检测结果没有显著性差异。通过62名检测人员对黄曲霉毒素免疫磁珠自动化处理方法与传统的免疫亲和柱净化法的评测,通过t-检验可知,99%的置信区间内,P值≥0.01,说明两种方法的测定值没有显著性差异,但是免疫磁珠自动化处理法测定结果的离散程度小于免疫亲和柱法,且全部的检测结果的|Z|均≤2。

    Abstract:

    The objection was to evaluate the applicability of an automated method for aflatoxins analysis by using Immunoaffinity Magnetic Bead - UPLC. The results showed that the average recovery of the mmunoaffinity Magnetic Bead was above 95%, and the relative standard deviation was less than 3.3%. The results of the quality control samples were within the range of the reference uncertainty. The method was accurate and reliable.T testing (sig. (bilateral) = 0.533) proved that there was no significant difference between two independent purification instruments.62 researchers evaluated two methods of purifications with immunomagnetic beads and immunoaffinity column. T testing has proved that the results of the two methods were no significant difference. However, the dispersion degree of the immunomagnetic bead automated processing method was smaller than that of the immunoaffinity column method. Moreover, the z-value of all the test were less than 2 (| Z | ≤2). Hence, this method can detect cereals and oils. In addition, the method is environmentally friendly, simply, and lower-cost, which greatly improved detection efficiency and level.

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李 丽,叶 金,轩志宏,刘洪美,陈梦泽,周明慧,吴 宇,王松雪.基于免疫磁珠黄曲霉毒素全自动净化方法适用性评价[J].粮油食品科技,2020,28(3):37-41.

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  • 在线发布日期: 2020-05-12
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