It is significant to understand the influence of insects among stored grain on the content of mycotoxin in the storage condition of non-mouldy and non-toxic. Aflatoxin B1 (AFB1) contents in the maize were detected after being infected by larvae and imagos of Ahasverus advena(Waltl) in different time. The results indicated that AFB1 content and mold quantity decreased significantly in the maize infected by the larvae and imagos of A. advena. Imagos were more effective in decreasing AFB1 content than larvae. The lower the broken kernels rate was, the more effective in decreasing AFB1 content. AFB1 content in maize decreased significantly with the insect densities increasing and time going.