The antioxidant activity and immunoregulationon of three kinds of fruit and vegetable zymotic fluid, which were named A, B and C, were researched. The effect of the three kinds of fruit and vegetable zymotic fluid on ROS level in cell was evaluated by AAPH-induced in HepG2 cells model. To study the immunomodulatory ability, concanavalin A and lipopolysaccharide were used to induce the proliferation of mouse T/B lymphocytes, and lipopolysaccharide was used to induce mouse peritoneal macrophages, detecting phagocytic ability and NO release. The results showed that the three kinds of fruit and vegetable zymotic fluid had strong ability to eliminate ROS in cells, A fruit and vegetable zymotic fluid can make intracellular ROS content as low as 55%. They can improve lymphocyte proliferation ability, phagocytic ability of peritoneal macrophages and promote NO release, A fruit and vegetable zymotic fluid has a maximum NO release of 4.06 μmol/L at dilution ratio of 104. These results indicated that the three kinds of fruit and vegetable zymotic fluid have good antioxidant activity and enhance immune function.