In order to optimize the preparation process conditions of peanut protein concentrate by Viscozyme L pretreatment combined with ethanol washing method, the influence of preparation process conditions on protein quantity percentage and extraction rate was investigated by single factor experiments and response surface methodology with peanut meal as the raw material. The results showed that the optimum conditions obtained were enzyme dosage 6.1 FBG/g, pH value 4.2, enzyme hydrolysis temperature 43 ℃ and time 4.5 h, respectively. Under the optimum conditions, the verification experiment values of protein quantity percentage and extraction rate were 73.21%±0.59% and 85.23%±0.67%, respectively. The difference between the predicted values and the verified values was less than 1%, which provided a new way for further development and utilization of peanut meal.