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黄曲霉毒素B1降解菌株的筛选鉴定
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Screening and identification of aflatoxin B1 degrading strains
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    摘要:

    黄曲霉毒素B1(AFB1)具有极强的毒性和致癌性,严重危害人和动物健康。通过富集培养、初筛和复筛从土壤中筛选到能降解黄曲霉毒素的菌株,鉴定降解效率最高菌株并研究其降解特征。结果表明,筛选出多株能降解AFB1菌株,其中菌株A12降解效率最高,通过形态学、生理生化特征及16S rRNA基因系统进化分析鉴定菌株A12为枯草芽孢杆菌,命名为Bacillus subtilis subsp. Inaquosorum A12。研究发现枯草芽孢杆菌A12上清液、菌悬液、胞内液能分别降解87.6%、17.3%和10.8%的AFB1,表明菌株A12分泌至胞外活性物质主导生物降解作用。菌株A12上清液降解AFB1的最适反应温度为37 ℃,最适pH为7.0。将该菌接种到AFB1污染的花生样品,能显著降低AFB1的含量。为枯草芽孢杆菌A12应用于AFB1的生物脱毒奠定了基础。

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    Aflatoxin B1 (AFB1) is highly toxic and carcinogenic, which seriously endangers human and animal health. The aflatoxin degradation strains were screened from the soil through enrichment culture, initial screening and rescreening, and the efficient degradation strain was identified and the degradation characteristics were studied. The results showed that the selected strains could degrade AFB1, and the strain A12 was the most efficient one, which was identified as bacillus subtilis, named as Bacillus subtilis subsp. Inaquosorum A12, by morphological, physiological and biochemical analyses and the evolution of 16S rRNA gene system. The degradation rate of supernatant, bacterial suspension, and intracellular fluid of A12 were 87.6%, 17.3% and 10.8% AFB1, respectively. The results indicated that degradation activity of strain A12 was located in extracellular fluid, and the optimal reaction temperature and pH value for degradation of AFB1 by supernatant were 37 ℃ and 7.0, respectively. The AFB1 content could be significantly reduced by inoculating strain A12 to peanut sample contaminated by AFB1. This study laid a foundation for the biological detoxification of AFB1 by strain A12.

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王明清,于丽娜,张初署,毕 洁,孙 杰,刘 宾.黄曲霉毒素B1降解菌株的筛选鉴定[J].粮油食品科技,2018,26(5):63-69.

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  • 在线发布日期: 2018-09-21
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