对高效液相色谱法测定全麦粉中呕吐毒素含量进行了研究,分析了前处理方法、提取方式和提取时间、标准曲线及色谱柱等因素对全麦粉中呕吐毒素含量测定结果的影响。结果表明：与多功能净化柱法相比，对于样品提取液色泽深的陈麦粉，采用0.1%吐温洗脱添加样品后的免疫亲和柱，样品处理干净,色谱图杂峰少，利于目标峰分离；与GB 5009.111—2016中的色谱测定条件相比，C18，5 m 4.6250 mm型号的色谱柱更适用于全麦粉中呕吐毒素含量的测定。
The content of deoxynivalenol (DON) in whole wheat flour was detected by high performance liquid chromatography (HPLC). The influence of pretreatment method, extraction mode and extraction time, standard curve and chromatographic column on the determination were analyzed. The results indicated that in contrast to the multifunctional purification column treatment method, the immuno-affinity column method used 0.1% of tween leachate sample solution for ageing flour with deep color, the sample was treated clean and the measured chromatogram had less peaks, which was beneficial to the separation of the target peak. Compared with the chromatographic conditions of GB 5009.111-2016, the chromatographic column of C18 and 5 m 4.6 250 mm was more suitable for the determination of the content of vomiting toxin in whole wheat flour.