粮油食品科技
燕麦蛋白组分分离提取及其SDS-PAGE电泳分析
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Separation and extraction of oat protein components and analysis by SDS-PAGE
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    摘要:

    通过单因素实验对燕麦蛋白组分的分离提取工艺进行了优化,并通过SDS-PAGE电泳对燕麦蛋白组分进行亚基分析。结果表明:燕麦清蛋白提取的最佳温度为40 ℃,球蛋白提取最佳盐浓度为7%,醇溶蛋白提取的最佳乙醇浓度为75%,谷蛋白提取的最佳碱浓度为0.05 mol/L,蛋白质提取率为83.1%。SDS-PAGE实验结果显示:燕麦清蛋白在10~100 kD范围内均有分布,燕麦球蛋白由2个亚基组成,分子量分别在97.4~100 kD和43~66.2 kD范围内,燕麦醇溶蛋白亚基大部分集中在18.39~40.72 kD之间,燕麦谷蛋白部分亚基分布在20.67~26.66 kD与43.29~50.80 kD之间。

    Abstract:

    The separation and extraction process of protein components in oat were optimized by single factor experiments and the protein subunit components of oat were analyzed by SDS-PAGE. The results showed that the optimal temperature for extraction of oat albumin was 40 ℃, the optimal salt concentration for globulin was 7%, the optimal ethanol concentration for prolamin was 75%, the optimal alkali concentration for glutenin was 0.05 mol/L, the extraction rate of oat protein 83.1%. The results of SDS-PAGE showed that the oat albumin distributed in the range of 10~100 kD; the oat globulin was composed of two subunits with molecular weight in the range of 97.4~100 kD and 43~ 66.2 kD respectively; the distribution range of most oat prolamin subunits was 18.39~40.72 kD; and the distribution range of some oat glutenin subunits was between 20.67~26.66 kD and 43.29~50.80 kD.

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王美玉,粱亚萍,王 愈,陈振家.燕麦蛋白组分分离提取及其SDS-PAGE电泳分析[J].粮油食品科技,2018,26(5):1-5.

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  • 在线发布日期: 2018-09-21
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