粮油食品科技

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组合抗生素抗性选育多杀菌素高产菌株
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    摘要:

    通过2种途径向菌株ASAGF W2引入链霉素、庆大霉素、利福平和氯霉素抗性,研究抗生素抗性筛选技术对选育多杀菌素高产菌株的作用效果。途径一是将4种抗生素的抗性通过含有抗生素的平板逐级引入,标记为非GYM组;途径二是将分离得到的抗性突变菌进行纯培养后通过抗生素平板引入下一种抗性,标记为GYM组,利用摇瓶发酵进行多杀菌素高产菌株的选育。结果显示非GYM组的方法是进行抗生素抗性筛选的较适途径;通过对高产菌株连续转接5次,最终获得4株遗传稳定的高产突变菌,其中突变菌13-8-1来自非GYM组,具有Str0.5Gen10双重抗性,多杀菌素平均发酵产量较出发菌株ASAGF W2提高了23.87%。利用抗生素抗性筛选技术选育多杀菌素高产菌株,简单易行且效果显著。

    Abstract:

    The effect of antibiotic-resistance screening on breeding of spinosad high-producing strains was researched by leading streptomycin, gentamicin, rifampicin and chloramphenicol into strain ASAGF W2 with two approaches.The first one was inducing different resistance to antibiotics through antibiotic plates successively, which was marked as non-GYM group. The second one was inducing next kind of resistance to antibiotic by antibiotic plates after the mutants was isolated and purified through streak plate method, which was marked as GYM group. All mutants were bred by the shake flask culture. The results show that non-GYM group is more suitable method for antibiotic resistance screening. Four spinosad high-producing strains have been obtained through five rounds screening and mutant 13-8-1 was obtained from isolates of antibiotic combination Str0.5Gen10 from non-GYM group and showed enhanced spinosad production by 23.87%, compared to original strain. The approach to screen spinosad high yield strains using multiple antibiotic-resistance screening is effective and easy in operation.

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王海霞,陈 园,王 超,黎 琪,陈 新,张晓琳.组合抗生素抗性选育多杀菌素高产菌株[J].粮油食品科技,2017,25(4):70-75.

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  • 在线发布日期: 2017-07-20
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