采用酶法水解麦麸水不溶性阿拉伯木聚糖制备低聚木糖，研究酶解温度、酶解时间和加酶量对低聚木糖含量及其糖组成、平均聚合度以及益生元活性的影响。结果表明：随着酶解时间由1 h延长至2 h、酶解温度由40 ℃增加至50 ℃和加酶量由0.125 g/L增加至1 g/L时，低聚木糖含量增加，平均聚合度减小，木二糖、木三糖和木四糖的相对含量比值减小;不同酶解条件下得到的低聚木糖培养青春双歧杆菌的菌体浓度含量均有不同程度的增加;青春双歧杆菌代谢不同酶解条件下得到的低聚木糖发酵液中代谢产物均含乳酸、乙酸和丙酸，乙酸含量最高，其产酸量变化趋势与菌体浓度和低聚木糖含量相一致，与低聚木糖平均聚合度相反。当酶解温度50 ℃，酶解时间2 h和加酶量1 g/L时，低聚木糖含量达到最大为101 mg/g，平均聚合度降至最小3.23，木二糖、木三糖和木四糖的相对含量比值也最小，菌体浓度增值倍数达到最大为5.65倍。
Using wheat bran insoluble arabinoxylan as raw materials for preparation of xylooligosaccharides by enzymatic hydrolysis, the effect of enzymolysis temperature, enzymolysis time and enzyme dosage on the content of xylooligosaccharides, sugar composition, average polymerization degree and prebiotic activity were studied. The results showed that the content of xylooligosaccharides increased, while the average degree of polymerization and the relative content ratio of xylose, xylose and xylose decreased when the enzymolysis time increased from 1 h to 2 h, the hydrolysis temperature increased from 40 ℃ to 50 ℃ and the amount of enzyme increased from 0.125 to 1 g/L. When Bifidobacterium adolescentis was cultured with xylooligosaccharides obtained under different enzymolysis conditions, the concentration of Bt increased in different extents. The metabolites of fermentation broth contained lactic acid, acetic acid and propionic acid, and the content of acetic acid was the highest. The trend of acid production was consistent with the concentration of bacteria and the content of xylooligosaccharides, while contrary to the average polymerization degree of xylooligosaccharides. The content of xylooligosaccharides reached to 101 mg/g, the average degree of polymerization decreased to 3.23, the relative content ratio of xylose, xylose and xylose was the smallest, and the multiplication of cell concentration was up to 5.65 times when the enzymolysis temperature was 50 ℃, the time of enzymolysis was 2h and the amount of enzyme was 1 g/L.