Determination of deoxynivalenol(DON) in wheat and its products by colloidal gold strips was very fast and accurate. Its recovery rate was between 86.67%～120%. Compared with the HPLC method, the results showed that there was no significant difference between the two methods in the detection of DON. The recovery rate was a little higher than HPLC. But the coefficient of variation was bigger. At low concentrations (<1500μg/kg), the rapid method had a linear relationship with the standard method: Y=1.0773X+83.786(Y represent the result by rapid method, X represent by HPLC method, R2=0.9637).