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DHA软胶囊中3种特征标志物的测定及其品质评价
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Determination of 3 kinds of characteristic markers in DHA soft capsules and its quality assessment
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    摘要:

    针对不同来源的DHA软胶囊,建立一种同时测定其3种特征标志物的检测方法,并结合其他理化指标进行品质的综合评价。建立的特征标志物测定方法为:样品经甲酯化衍生化处理后,采用外标法对不同DHA软胶囊中DHA、EPA、亚油酸的甲酯进行检测,脂肪酸甲酯经换算得到相应脂肪酸的含量。4个品牌的产品性状、酸价、过氧化值、砷均符合国家相关标准。特征标志物测定的方法学验证结果为:DHA甲酯、EPA甲酯及亚油酸甲酯的精密度RSD分别为1.39%、1.43%、0.5%;线性范围分别为0.3~2.4mg/mL、0.2~2.4mg/mL、0.1~1.6mg/mL,相关系数分别为1.000、0.9999、1.000,线性关系均良好;平均回收率分别为96.9%~101.4%、97.5%~100.3%、95.4%~99.6%,准确度较高;定量限浓度分别为0.08、0.04、0.03 mg/mL;24h内的稳定性数据(RSD)分别为3.19%、3.65%、3.02%,说明稳定性好。DHA软胶囊的评价结果表明:3#的酸价较高,崩解较慢;2#的DHA和亚油酸含量较低;0#DHA软胶囊的亚油酸含量明显高于其他品牌。与其他理化指标相比,3种特征标志物在4个品牌产品之间差异较明显,可较好地反应关键品质变化。

    Abstract:

    A method was established for the simultaneous determination of 3 kinds of characteristic markers in DHA soft capsules from different sources. The other physicochemical indexes were comprehensive assessed. The method was: after methyl esterification and derivatization, the methyl esters of DHA, EPA and linoleic acid in different DHA soft capsules were detected by external standard method, and then the contents of the corresponding fatty acid were obtained by conversion. The product characteristics, acid value, peroxide value and arsenic of 4 samples from different companies were in line with relevant national standards. The results of verification test for the determination of DHA methyl ester, EPA methyl ester and linoleic acid methyl ester were as follows: the precisions were 1.39%, 1.43%, 0.5%; the linear ranges were 0.3~2.4mg/mL, 0.2~2.4mg/mL, 0.1~1.6mg/mL; the correlation coefficients were 1.000, 0.9999 and 1.000, which showed good linear relationship; the average recovery ranges were 96.9%~101.4%, 97.5%~100.3%, 95.4%~99.6% ,respectively, which showed that the method had high accuracy; the concentrations of the limit of quantitation (LOQ) were 0.08, 0.04, 0.03mg/mL, respectively. The stability data (RSD) within 24h were 3.19%, 3.65%, 3.02%, which indicated that the sample had good stability.The evaluation results of DHA soft capsule showed that the 3# had high acid value and slow disintegration, the 2# had low content of DHA and linoleic acid, and the 0# had the highest content of linoleic acid among the samples. Compared with other physicochemical indicators, the difference among 4 brand products on the 3 kinds of characteristic markers were larger and the markers were suitable to reflect the key quality.

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杜双有,宋慧颖,方静,沈文忠,李福高. DHA软胶囊中3种特征标志物的测定及其品质评价[J].粮油食品科技,2017,25(2):51-55.

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  • 在线发布日期: 2017-03-23
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