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皇菊水溶性色素树脂纯化工艺及其稳定性研究
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Study on resin purification process and stability of water-soluble pigments from chrysanthemum
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    以热水浸提法得到的皇菊色素为研究对象,利用DM-28大孔树脂纯化皇菊色素,优化其静、动态纯化工艺条件,并对纯化后的皇菊色素进行稳定性分析。结果表明,静态吸附—解吸最佳条件为:吸附平衡时间3h,样液浓度为色素原液被稀释12倍(即A390=0.361),样液pH 2.5,吸附温度30℃,在pH 3.0、50%乙醇溶液条件下进行解吸;动态吸附—解吸最适工艺条件为:上样浓度为原液稀释12倍(即A390=0.361),上样流速2.0mL/min,以流速1.0 mL/min、80%乙醇溶液进行洗脱。纯化后色素色价为22.5,是纯化前的3.65倍。DM-28大孔树脂具有良好的吸附性能,皇菊色素相对来说比较稳定,对外界条件不敏感,可以考虑作为一种天然食品色素应用到食品工业中。

    Abstract:

    The pigment extracted from chrysanthemum by hot water method was selected as the investigate object,and purified by DM-28 macroporous resin. Static-dynamic purification conditions of the water-soluble pigments of chrysanthemum were optimized. The stability of the pigment was analyzed. The results showed that the optimal static adsorption-desorption conditions were as follow: adsorption equilibrium time was 3 h, the concentration of sample was the fluid diluted 12 times from the original (namely A390=0.361), sample pH was 2.5, adsorption temperature was 30℃, with pH 3.0, 50% ethanol as eluent for desorption. The optimal dynamic adsorption conditions were: sample concentration was A390=0.361, the sample flow rate was 2.0mL/min, eluted with the flow rate of 1.0mL/min, 80% ethanol. The color value of the purified pigment was 22.5, which was 3.65 times of the former. DM-28 macroporous resin show better comprehensive adsorption property. The pigment was of relatively better stability, could be used as a kind of natural food additive in the food industry.

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胡洁芳,洪艳平,刘玉珍,曾璟,万琴,胡远诚,张敏.皇菊水溶性色素树脂纯化工艺及其稳定性研究[J].粮油食品科技,2017,25(2):44-50.

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  • 在线发布日期: 2017-03-23
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