The aflatoxin B1 and deoxynivalenol in corn was quantitatively detected by portable mycotoxin rapid quantitative detector， the result was compared with that by enzyme linked immunosorbent assay. The results showed that the detection limit of aflatoxin B1 was 1.75μg/kg by rapid quantitative detection card. The accuracy had no significant difference between the two methods； weight measurement range of the coefficient of variation between 7%～11.2%， the average coefficient of variation was 8.7%； no significant difference among the quantitative detectors. The detection limit was 0.078 mg/kg by deoxynivalenol rapid quantitative detection card； no significant difference among the quantitative detectors. The portable quantitative detector has the advantages of simple， fast， low cost， can realize site operation and upload real-time data and recording monitoring site location， which is worth promotion， particularly being used in the health survey， research， screening and other field.