The characteristic indexes， physical-chemical indexes， and main food safety indexes of hempseed oil which were produced in Guangxi were determined. The results showed that the refractive indexes (n40) were between 1.4698～1.4702， relative densities (d2020)0.9230～0.9261， iodine values 162.8～168.2g/100g， saponification values 189.9～197.0mg/g. 12 kinds of fatty acid compositions， including myristic acid，palmitic acid， palmitoleic acid， heptadecanoic acid， cis-10-Heptadecenoic acid， stearic acid， oleic acid， linoleic acid， linolenic acid， arachidic acid， cis-11-Eicosenoic acid and behenic acid， could be detected in hempseed oil by gas chromatography. The total amount of unsaturated fatty acids accounted for 83.6%～90.4%， and the proportion of polyunsaturated fatty acids were 71.6%～79.1%. The unsaturated fatty acids were mainly oleic acid， linoleic acid， linolenic acid， accounting for 9.6%～14.7%， 52.2%～58.2% and 15.2%～23.9% respectively. The total saturated fatty acids accounted for 9.3%～12.0%. The main components of the saturated fatty acids were the palmitic acid and stearic acid. The results of characteristic indexes showed that hempseed oils were rich in polyunsaturated fatty acids， and were rich in linoleic acid and linolenic acid simultaneously， which had high nutritional and health value. The results of physical-chemical indexes and food safety indexes showed that the contents of moisture and volatile matter， acid value， benzo(α)pyrene and lead should be monitored and controlled， especially for acid value and benzo(α)pyrene.