The stability of the continuous subculture of lead-resistant lactic acid bacteria JT1 in the optimized medium (M1) and MRS medium added with konjac flour (M2) was researched in the aspects of the phenotypic characteristics and probiotic characteristics. The results showed that the form of lead-resistant lactic acid bacteria JT1 did not changed in both M1 and M2 after the 1000 generations. The modality of colonies was opaque white protuberance with smooth surface and consistent with the original isolated colony. Continuous culture didn't change the total biomass in culture cycle and the living bacteria number at the end of the culture cycle. The tolerance of JT1 kept stable in artificial gastric juice(pH 2.5), artificial intestinal juice(pH 8.0) and 0.3% bile salts.In particular, the specific function of adsorption of Pb2+ was nearly not affected during the passage, and the adsorption rate was basically stable at around 60%. These results provide the reliable basis for the stainable production of lead-resistant lactic acid bacteria JT1.