A new deoxynivalenol (DON) detection method which based on the morphological characteristic of fusarium that produces DON in wheat was preliminary set up. Fusarium conidiophores were the detected target of this method. The spores in the samples were oscillated in water, filtered with 100 mesh filter cloth and the spores in the filtrate were microscopic tested using a microscope. The whole process takes three minutes. The relative standard deviation (RSD) of the wheat positive samples test (n=6) with different infection levels was 7.1% ～21.6%. 72 wheat samples collected from main wheat producing areas in China were inspected by this method, and the HPLC method was used as the control. The results showed 〖JP3〗that fusarium conidiophores were nearly not found in 46 samples, account for 63.9 % of the total samples, in which DON concentration range were 0～792.4 μg/kg which did not exceed the limitation of national standard 1000μg/kg. The fusarium conidiophores were detected in 26 samples, in which DON were not exceed the limitation in 6 samples, and the growth of fusarium was positively related to the yield of DON. The small batch of wheat can be quickly screened out by this method whether exceeding the DON limitation standard.