Using crude flavonoids extracted from red kidney beans as the raw materials, the adsorption and desorption efficiencies of flavonoids with six different macroporous resins were compared,and XDA-1 resin was selected as the most efficient adsorbent as it had good adsorption and desorption capacity for flavonoids from red kidney beans. The antioxidation effect of flavonoids extract from red kidney beans on edible oils and fats (lard and peanut oil) was investigated. The results showed that the optimal separation condition was: sample liquid concentration 1.5 mg/mL, adsorption flow rate 2 BV/h, sample pH value 6, sample volume 3BV, elution speed 2 BV/h, and ethanol volume fraction 60%, elution volume 3BV. Under the optimal separation condition the flavonoids yield and purity were 73.86% and 22.65%, respectively. The flavonoids extract can effectively defer the lipid oxidation, and moreover its antioxidant effect on lard was better than that on peanut oil.