A rapid detection method was established for the determination of 53 kinds of three varieties of pesticide residue, organochlorine, organophosphorus and pyrethroid, in rice by gas chromatography- electron capture detector(GC-ECD). The sample was extracted by acetonitrile and purified by N- propyl Ethylenediamine and C18. The 53 pesticides showed a good linear relationship in the concentration range of 0.001～0.1μg/mL. The recovery rates were 85.2%～97.8%, the relative standard deviations were 0.9%～4.1%, the detection limits were 0.001～0.006 mg/kg.The method is high sensitivity, accurate and reliable, which can meet the requirements for determination of pesticide residues in rice.