In order to confirm the best extract process conditions, the effect of the factors and the interactions among them on the extraction of flavonoids in the peel of Henan tiegun yam was researched by response surface method. The factors were extraction time, solid-liquid ratio, sieving mesh number and ethanol volume concentration. The optimal factors confirmed by software Minitab 16 were adjusted to ethanol volume fraction 79%, solid-liquid ratio 0.023g/mL, extraction time 75 min and sieving mesh number 120 mesh. The average yield was 0.3382mg/g determined by verification test, close to the predicted value. The test data was stable, which indicated that the regression model could predict the yield rate of flavonoids in the peel of Henan tiegun yam.