Two methods in AOCS were compared to determine tocopherols and tocotrienols in vegetable oils. The results showed that the high performance liquid chromatography-fluorescence detection (HPLC-FLD) was more sensitive than high performance liquid chromatography- ultraviolet (HPLC-UV), which has better recovery rate and accuracy, lower detection limits and higher resistance to interference of impurity. The results of determining tocopherols and tocotrienols in 12 kinds of vegetable oils by HPLC-FLD were all in the reference range of the literatures.