To explore the changes of the major nutrient contents in maize which was contaminated by Aspergillus sp. , the fungi pollution analog system was used to treat two varieties of maize, Xianyu 335 and Jiyuan NO. 1, for 7 days with aflatoxin producing fungal spore suspension. The results showed that the contents of protein and total amino acid of both varietied of maize were decreased. The changes of fat and sugar were related to maize variety. The content of fat in Xianyu 335 did not change, while total sugar content increased by 38.5%. The content of fat in Jiyuan NO. 1 decreased by 32.2% compared with control group, and the total sugar content decreased by 44.8 %. The contents of VA in both varieties of maize were reduced after being treated by the contaminated suspension, while the contents of VE and VB2 increased respectively; the change of content of VB1 was related to maize variety. Fungi changed major nutrient in maize in different degree which was related to maize variety. The contaminated maize should be evaluated for nutrient contents and safety, and graded before being used.