Subcritical water treatment was used to extract favonoids from cole pollen. The optimal conditions were ascertained by single factor test and orthogonal test. The results showed that the optimal conditions of subcritical water extraction were as following: the ratio of material to water 1:25 g/mL, the time 20 min, the temperature 120 °C and the pressure 1.2 MPa. Under the conditions, the yield of favonoids could reach up to 4.12% ±0.05%