The application of transverse ventilation system in large warehouse (60×21) for wheat storage was studied, and the system was designed. The air velocity, volume, pressure and resistance of the ventilation system and the static pressure in the grain bulk were detected. The results showed that both the total resistance of ventilation system and the unit profile resistance of grain bulk increased along with the increase of the unit aeration volume, which should be no more than 7m3/h•t during the aeration application. The coefficient of variation of static pressure in the grain bulk was small, which reflected high uniformity of airflow distribution.