The effects of different kinds of soybean milk on mice under normal diet and high fat feed conditions were researched. Four different kinds of soybean milk, including non-transgenic home-made soybean milk F, transgenic home-made soybean milk G and two kinds of common commercial ready-made soybean milk K and M, were selected to feed the C57 mice by gavage. Normal and high-fat diets were added, respectively. The executions were performed after 10 weeks. The weight growth rate, liver coefficient and colon length of the mice were measured. The concentrations of GLU, TG, CHOL, HDL-C and LDL-C in mice serum were determined by a biochemical analyzer. Human colon cancer cell line HCT-116 was used to investigate the influences of different soybean milks on the proliferation of cells and accumulation of lipid droplets. Results showed that the level of blood TG in mice was reduced by all of the soybean milks. Commercial ready-made soybean milk M could lower the concentrations of GLU and CHOL. The cell proliferation were significantly decreased by the soybean milks, however, the decrease were non-significant when the proliferation were induced by the oleic acid. Same results were found in the accumulations of lipid droplets. Different soybean milks cocld bring different effects on concentrations of GLU, TG, and CHOL etc in mice serum for their various contents of nutritional and functional factors. The commercial ready-made soybean milk was more conducive to reduce the adverse impact brought by high fat feed than the home-made soybean milks.