Triglycerides in palm oil and animal fats such as lard, beef tallow and suet were investigated by HPLC/Q-TOF. The results showed that the composition and content of triglycerides in each oil or fat were different. 16, 19, 15 and 13 triglycerides were identified in palm oil, lard, beef tallow and suet, respectively; in which the content of trisaturated glycerides were 9.13%, 1.46%, 13.75% and 9.05%, separately. PPO was the major triglyceride (34.41%) in palm oil, followed by PLO+SLL, PPL and PPP. OLS, PLO+SLL, POS and POO with content of 67.65% were the dominant triglycerides in lard. In beef tallow, the content of POS and OLS was 40.17%, the content of PPO、SSL and SOS was relatively high. SSL、POO、SOS and POS in suet was 73.12%. Meanwhile, 4 oils and fats were statistically analyzed using principal component analysis (PCA).