The purification of roselle anthocyanins was studied. The most suitable resin for roselle anthocyanin purification was HPD-100 macroporous resin, which was obtained by screening five kinds of macroporous resins: X-5, DA-201, HPD-100, D101 and AB-8. The purification conditions for HPD-100 macroporous resin were optimized by single factor and orthogonal experiments, which were: the sample′s concentration 1.5 mg/mL, flow rate 2 mL/min, pH 3. The adsorption rate can reach 91.988%. The optimal desorption process conditions were eluent concentration 70%, elution velocity 1.75 mL/min, dosage of eluent 60 mL. The desorption rate can reach 92.144%. After the purification，the color value of roselle anthocyanins was 38.5. Compared before and after the purification, the chromatograms by HPLC showed that roselle red pigment mainly contained five components. The cornflower anthocyanin content in the pigment increased significantly after being purified.