The mycotoxins pollution is one of the main factors that induce food safety problems. At present, the popular methods for determination of mycotoxins in cereals and oils are mainly thin-layer chromatography, high performance liquid chromatography, supercritical fluid chromatography, gas chromatography-mass spectrometry, immunosensor, ELISA kits and rapid test card etc. Electrochemical immunosensor has great potentialities in rapid detection, online detection and on-site determination for 〖JP2〗mycotoxins in foods because of the advantages such as rapid, sensitive and specificity. The reaction mechanism, classification, antigen/antibody immobilization of electrochemical immunosensor were summarized, the applications of aflatoxin immunosensors, ochratoxin immunosensors, fumonisin immunosensors, deoxynivalenol immunosensors and zearalenone immunosensors in determination for mycotoxins in cereals and oils were briefly described. The present problems were analyzed and suggestions were given in the end.