The organophosphorus pesticides residues in carrot juice was determined by stir bar sorptive extraction (SBSE) combining with liquid chromatography- tandem mass spectrometry. The sample was extracted by SBSE. After being desorbed, the sample was performed on ZORBAX Eclipse Plus C18 column. The analysis was carried out in the multiple reaction monitoring mode and external standard method was used for quantification. The calibration curve of the three organophosphorus pesticides showed a good linear relationship as concentrations in the range of 0.5~50 μg/L with the correlation coefficient r=0.995 3～0.999 9, the detection limit of this method was 0.5 μg/kg. In different concentrations, the average recovery rate of this method was 86.8%～95% and the coefficient of variation 4.3%～12.8%.