在总氮相等条件下优化了红霉素工业发酵培养基的速效氮源和迟效氮源比例，同时尝试性地引入羽毛蛋白胨作为新型速效氮源。实验结果表明，迟效氮源与速效氮源比值为3.9时，即羽毛蛋白胨浓度为6 g/L、黄豆饼粉为33 g/L时，红霉素效价最高为11 059 U/mL。与玉米浆相比，羽毛蛋白胨具有质量稳定的特点，从而可避免玉米浆质量不稳定引起的红霉素效价波动。采用优化后的培养基，比较了传统pH反馈补糖工艺和pH结合呼吸熵（RQ）反馈补糖工艺。结果表明，pH结合RQ反馈补糖工艺下的红霉素效价达到11 915 U/mL，比传统pH反馈补糖工艺的效价提高了7.7%，而新补糖工艺下的补糖总量降低了40%。
The ratio of available nitrogen source to slow release nitrogen source in erythromycin industrial fermentation medium were optimized under the condition of equal proportion of total nitrogen. Meanwhile feather peptone was tentatively used as a new type of available nitrogen source. The experimental results showed that, when the optimal ratio of available nitrogen source to slow release nitrogen source was 3.9, (the concentration of feather peptone 6 g/L and the soybean meal 33 g/L)，erythromycin titer reached to 11 059 U/mL. Compared with corn steep liquor, feather peptone has the characteristic of stable quality, thus erythromycin titer fluctuation caused by quality instability of corn steep liquor can be avoided. Using the optimized medium, the traditional pH-feedback glucose feeding was compared with pH-RQ-feedback glucose feeding. The results showed that the erythromycin titer reached to 11 915 U/mL with pH-RQ-feedback glucose feeding, which was 7.7% higher than that with the traditional pH-feedback glucose feeding, moreover the total glucose amount fed to fermenter was decreased by 40%.