The process of CTAB-assisted extraction of pectin from blood pomelo peel was optimized. Based on the single-factor experiments, selecting concentration of CTAB, pH, temperature and time as independent variables and the extraction yield of pectin as response value, the effects of each independent variable and their interactions on the extraction yield were studied by Box-Behnken design. The quadratic multinomial model of each factor and the yield was set by Design-Expert 8.05b software.The results showed that the optimal extraction conditions of pectin from blood pomelo peel were: CTAB concentration 0.62%, pH 2.1, temperature 56 ℃ and time 93 min. The yield of pectin could be up to 20.85% under the optimal extraction condition, the relative error was 0.28% compared to the predictive value, which indicated the feasible model fitted well with the experimental data. The pectin showed a viscosity of 58.2×10-3 Pa·S, content of methoxy group 6.47%, degree of esterification 38.9%, ash content 0.82%, content of total galacturomic acid pectin 85.7%, which accorded with the standard of QB2484-2000. The pectin was a kind of low methoxy pectin and could be used as thickener.