High F ratio oligopeptide mixture (F ratio 24.6) was prepared by enzymatic hydrolyzing peanut meal with alkaline protease Alcalase and papain. The function of relieve fatigue of the mixture was tested. The results showed that the rolling stick time of the mice which were given a dose of high F ratio oligopeptide by intragastric administration increased significantly, the content of serum urea nitrogen (SUN) decreased significantly, the content of hepatic glycogen and muscle glycogen increased in some degree. The high F ratio oligopeptide mixture from peanut meal had significant relieve fatigue effect.