The effect of factors on the extraction of camphor seed oil by ultrasonic was studied. The extraction techniques were optimized by Box-Behnken design in response surface method on the basis of single factor experiments. The optimum extraction conditions were ultrasonic temperature 70 ℃, ultrasonic treatment time 32 min, the ratio of material to solvent 1∶21 g/mL, the extraction rate under the condition can reach 38.12%, which was in good agreement with the model predicted value and can be applied in industrial production.